Antibody Bd Pharmingen™ Leukocyte Activation

Features

• Ready-to-use polyclonal cell activation mixture with phorbol ester PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitor BD GolgiPlug™ (brefeldin A)
• The most critical step for detection of intracellular accumulations of cytokines by intracellular staining is the isolation and activation of a cell population to induce production of the cytokine of interest
• This mixture may be utilized to elicit a primary cytokine response from T cells
• Stimulation of cells using this cocktail results in cytokine production that is localized in the rough endoplasmic reticulum of cytokine -producing cells
• The increased accumulation of cytokine protein levels in cells thus enhances detectability of cytokine-producing cells by immunofluorescent staining and flow cytometric analysis
• The contents of each 100 ?l vial is sufficient for treating up to 50 ml of cell cultures (at ~10e6 cells/ml); therefore one can stimulate ? 5 × 10e7 cells